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Vanadium in PDB 1b8j: Alkaline Phosphatase Complexed with Vanadate

Enzymatic activity of Alkaline Phosphatase Complexed with Vanadate

All present enzymatic activity of Alkaline Phosphatase Complexed with Vanadate:
3.1.3.1;

Protein crystallography data

The structure of Alkaline Phosphatase Complexed with Vanadate, PDB code: 1b8j was solved by K.M.Holtz, B.Stec, E.R.Kantrowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.90
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 195.410, 167.810, 76.520, 90.00, 90.00, 90.00
R / Rfree (%) 17.7 / 19.6

Other elements in 1b8j:

The structure of Alkaline Phosphatase Complexed with Vanadate also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Zinc (Zn) 4 atoms

Vanadium Binding Sites:

The binding sites of Vanadium atom in the Alkaline Phosphatase Complexed with Vanadate (pdb code 1b8j). This binding sites where shown within 5.0 Angstroms radius around Vanadium atom.
In total 2 binding sites of Vanadium where determined in the Alkaline Phosphatase Complexed with Vanadate, PDB code: 1b8j:
Jump to Vanadium binding site number: 1; 2;

Vanadium binding site 1 out of 2 in 1b8j

Go back to Vanadium Binding Sites List in 1b8j
Vanadium binding site 1 out of 2 in the Alkaline Phosphatase Complexed with Vanadate


Mono view


Stereo pair view

A full contact list of Vanadium with other atoms in the V binding site number 1 of Alkaline Phosphatase Complexed with Vanadate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:V102

b:42.4
occ:1.00
V A:SVA102 0.0 42.4 1.0
O2 A:SVA102 1.7 47.5 1.0
O3 A:SVA102 1.7 41.5 1.0
OG A:SVA102 1.7 34.5 1.0
O1 A:SVA102 1.8 44.8 1.0
O4 A:SVA102 1.9 41.6 1.0
CB A:SVA102 2.7 8.6 1.0
ZN A:ZN450 3.0 25.1 1.0
ZN A:ZN451 3.1 20.1 1.0
N A:SVA102 3.6 6.7 1.0
CE1 A:HIS412 3.6 21.2 1.0
O A:HOH1473 3.7 11.5 1.0
CA A:SVA102 3.7 8.1 1.0
NH1 A:ARG166 3.8 23.5 1.0
NE2 A:HIS412 3.9 16.9 1.0
O A:HOH1471 3.9 19.2 1.0
NH2 A:ARG166 3.9 33.5 1.0
OD1 A:ASP327 4.1 15.3 1.0
NE2 A:HIS370 4.1 16.8 1.0
O A:HOH1028 4.2 56.2 1.0
OD2 A:ASP327 4.2 17.9 1.0
CZ A:ARG166 4.4 33.1 1.0
CG A:ASP327 4.4 19.6 1.0
OD1 A:ASP51 4.4 23.4 1.0
OD2 A:ASP51 4.6 22.7 1.0
O A:HOH1124 4.6 58.2 1.0
C A:ASP101 4.7 6.5 1.0
NE2 A:HIS331 4.8 17.4 1.0
OD2 A:ASP369 4.8 16.1 1.0
ND1 A:HIS412 4.9 24.1 1.0
CG A:ASP51 4.9 20.2 1.0
CE1 A:HIS370 5.0 13.8 1.0
C A:SVA102 5.0 8.9 1.0
O A:HOH1363 5.0 53.2 1.0

Vanadium binding site 2 out of 2 in 1b8j

Go back to Vanadium Binding Sites List in 1b8j
Vanadium binding site 2 out of 2 in the Alkaline Phosphatase Complexed with Vanadate


Mono view


Stereo pair view

A full contact list of Vanadium with other atoms in the V binding site number 2 of Alkaline Phosphatase Complexed with Vanadate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:V102

b:34.7
occ:1.00
V B:SVA102 0.0 34.7 1.0
OG B:SVA102 1.7 42.2 1.0
O1 B:SVA102 1.7 36.2 1.0
O3 B:SVA102 1.7 36.8 1.0
O2 B:SVA102 1.8 41.3 1.0
O4 B:SVA102 1.9 33.4 1.0
CB B:SVA102 2.6 8.1 1.0
ZN B:ZN450 3.0 27.4 1.0
ZN B:ZN451 3.1 23.3 1.0
N B:SVA102 3.4 8.0 1.0
CE1 B:HIS412 3.6 27.7 1.0
CA B:SVA102 3.6 11.2 1.0
O B:HOH1320 3.6 45.3 1.0
O B:HOH1330 3.7 53.6 1.0
NH1 B:ARG166 3.8 31.4 1.0
NE2 B:HIS412 3.9 23.2 1.0
NH2 B:ARG166 3.9 35.5 1.0
O B:HOH1409 3.9 20.8 1.0
NE2 B:HIS370 4.1 10.3 1.0
OD1 B:ASP327 4.2 29.4 1.0
O B:HOH1224 4.3 66.4 1.0
OD2 B:ASP327 4.4 26.8 1.0
OD1 B:ASP51 4.4 28.8 1.0
CZ B:ARG166 4.4 33.0 1.0
CG B:ASP327 4.5 25.6 1.0
C B:ASP101 4.5 7.2 1.0
OD2 B:ASP51 4.6 24.9 1.0
OD2 B:ASP369 4.8 12.8 1.0
C B:SVA102 4.8 10.8 1.0
ND1 B:HIS412 4.8 23.4 1.0
CE1 B:HIS370 4.9 11.8 1.0
NE2 B:HIS331 4.9 18.6 1.0
CG B:ASP51 4.9 24.6 1.0
CA B:ASP101 5.0 7.9 1.0

Reference:

K.M.Holtz, B.Stec, E.R.Kantrowitz. A Model of the Transition State in the Alkaline Phosphatase Reaction. J.Biol.Chem. V. 274 8351 1999.
ISSN: ISSN 0021-9258
PubMed: 10085061
DOI: 10.1074/JBC.274.13.8351
Page generated: Wed Dec 16 02:30:06 2020

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